[04:21:30] kennethsulak leaves the room [09:10:22] samcandio leaves the room [09:18:51] kaseycantwell leaves the room [09:22:41] DIVE03 test message from Okeanos Explorer [09:32:20] EX2206_DIVE03 ROV powered off [09:34:44] EX2206_DIVE03 ROV powered off [09:46:28] JavierCristobo leaves the room: Replaced by new connection [09:48:06] EX2206_DIVE03 ROV powered off [09:50:44] EX2206_DIVE03 ROV powered off [10:03:03] daphnecuvelier leaves the room [10:18:45] EX2206_DIVE03 ROV Launch [10:26:58] EX2206_DIVE03 ROV on Surface [10:27:40] EX2206_DIVE03 ROV Descending [10:28:31] LAT : 38.014108 , LON : -26.782627 , DEPTH : 19.6366 m, TEMP : 22.64826 C, SAL : 36.23456 PSU, DO : 7.26736 mg/l, TURBIDITY : 0.7631 FTU [10:34:11] LAT : 38.015042 , LON : -26.781902 , DEPTH : 54.0611 m, TEMP : 19.8646 C, SAL : 36.20546 PSU, DO : 7.7839 mg/l, TURBIDITY : 0.8669 FTU [10:38:55] joanaxavier leaves the room [10:39:22] ROVs hanging out in water column for ~~20 min to conduct some USBL troubleshooting [10:40:09] joanaxavier leaves the room [10:43:14] LAT : 38.015644 , LON : -26.782318 , DEPTH : 54.7248 m, TEMP : 18.35531 C, SAL : 36.17369 PSU, DO : 7.79739 mg/l, TURBIDITY : 0.8913 FTU [10:45:12] daphnecuvelier leaves the room [10:48:46] LAT : 38.015614 , LON : -26.781767 , DEPTH : 53.8713 m, TEMP : 18.38413 C, SAL : 36.17347 PSU, DO : 7.79949 mg/l, TURBIDITY : 0.8852 FTU [11:00:31] LAT : 38.016028 , LON : -26.781463 , DEPTH : 54.9265 m, TEMP : 18.46158 C, SAL : 36.17731 PSU, DO : 7.78235 mg/l, TURBIDITY : 0.8974 FTU [11:05:31] LAT : 38.016054 , LON : -26.781485 , DEPTH : 55.0218 m, TEMP : 18.62386 C, SAL : 36.17964 PSU, DO : 7.79445 mg/l, TURBIDITY : 0.8913 FTU [11:10:32] LAT : 38.015989 , LON : -26.78146 , DEPTH : 54.7144 m, TEMP : 18.40775 C, SAL : 36.17527 PSU, DO : 7.79362 mg/l, TURBIDITY : 0.9035 FTU [11:14:07] daphnecuvelier leaves the room [11:28:45] LAT : 38.016019 , LON : -26.781452 , DEPTH : 55.0918 m, TEMP : 17.5812 C, SAL : 36.15238 PSU, DO : 7.69308 mg/l, TURBIDITY : 0.9158 FTU [11:33:46] LAT : 38.016067 , LON : -26.781474 , DEPTH : 54.9558 m, TEMP : 18.05652 C, SAL : 36.16313 PSU, DO : 7.77442 mg/l, TURBIDITY : 0.9219 FTU [11:33:50] EX2206_DIVE03 ROV powered off [11:33:52] EX2206_DIVE03 ROV powered off [11:33:53] EX2206_DIVE03 ROV powered off [11:37:35] Good morning Sam and all of you. Can't call in, but will try to chime in to the chat as we go. Happy hunting! [11:38:47] LAT : 38.016122 , LON : -26.781352 , DEPTH : 69.4675 m, TEMP : 16.85821 C, SAL : 36.12916 PSU, DO : 7.59871 mg/l, TURBIDITY : 0.9096 FTU [11:38:57] Morning Allen! [11:39:15] That sounds good Adrienne. Adjusting if necessary. [11:39:35] I missed what will be the depth after +10 off bottom [11:39:43] 1910 ish? [11:39:52] 1200 [11:40:03] Thanks. to you both. [11:41:46] we will be at the first transect around 1900 m in about an hour [11:41:57] daphnecuvelier leaves the room [11:42:17] Allen - sent you a second email about benthic fish eggs. Not much know in deep sea [11:43:47] LAT : 38.016104 , LON : -26.781282 , DEPTH : 221.421 m, TEMP : 14.89658 C, SAL : 35.99729 PSU, DO : 7.06016 mg/l, TURBIDITY : 0.9402 FTU [11:44:02] michaelvecchione leaves the room [11:47:45] isabelmoyer leaves the room [11:48:01] EX2206_DIVE03 ROV Descending [11:48:12] michaelvecchione leaves the room [11:48:48] LAT : 38.016165 , LON : -26.781191 , DEPTH : 378.4249 m, TEMP : 13.40761 C, SAL : 35.76274 PSU, DO : 6.6202 mg/l, TURBIDITY : 0.9463 FTU [11:49:17] kennethsulak leaves the room [11:49:45] ashleymarranzino leaves the room [11:50:17] michaelvecchione leaves the room [11:52:38] adriennecopeland leaves the room [11:53:49] LAT : 38.016229 , LON : -26.781134 , DEPTH : 533.7775 m, TEMP : 11.93123 C, SAL : 35.57475 PSU, DO : 6.2836 mg/l, TURBIDITY : 0.9463 FTU [11:58:37] taraluke leaves the room [11:58:49] LAT : 38.016232 , LON : -26.781032 , DEPTH : 690.1724 m, TEMP : 10.5528 C, SAL : 35.46273 PSU, DO : 5.99626 mg/l, TURBIDITY : 0.9463 FTU [12:03:49] LAT : 38.016426 , LON : -26.781032 , DEPTH : 835.9318 m, TEMP : 9.67588 C, SAL : 35.5382 PSU, DO : 5.76948 mg/l, TURBIDITY : 0.9402 FTU [12:05:46] michaelvecchione leaves the room [12:08:50] LAT : 38.016365 , LON : -26.781039 , DEPTH : 990.1374 m, TEMP : 9.36687 C, SAL : 35.68415 PSU, DO : 5.75888 mg/l, TURBIDITY : 0.9402 FTU [12:10:35] johnreed leaves the room [12:11:13] michaelvecchione leaves the room [12:11:25] I am particularly interested in sampling any comb jelly that has 4 horn-like projections. Here is a photo: https://www.teamorca.org/images/image011.jpg?crc=302749667 [12:13:13] adriennecopeland leaves the room [12:13:50] LAT : 38.016245 , LON : -26.781029 , DEPTH : 1147.6613 m, TEMP : 7.33635 C, SAL : 35.40306 PSU, DO : 6.57405 mg/l, TURBIDITY : 0.9402 FTU [12:14:13] I'm not hearing any audio at all yet. Is the problem at my end? [12:15:49] @Tara -- No audio here either. Probably mics are switched off. [12:16:28] Thanks @Mike Just making sure! [12:16:41] I have audio now [12:18:50] LAT : 38.016091 , LON : -26.780905 , DEPTH : 1300.5054 m, TEMP : 6.52358 C, SAL : 35.31628 PSU, DO : 7.00657 mg/l, TURBIDITY : 0.9341 FTU [12:19:33] allencollins leaves the room [12:21:38] adriennecopeland leaves the room: Replaced by new connection [12:22:58] what is bottom target depth for this dive? [12:23:51] LAT : 38.016208 , LON : -26.781049 , DEPTH : 1445.8301 m, TEMP : 5.41017 C, SAL : 35.14392 PSU, DO : 7.48386 mg/l, TURBIDITY : 0.9341 FTU [12:24:30] @Ken -- This is a midwater dive. 1st transect will be ca. 10 m above bottom at ca. 1900 m. [12:28:52] LAT : 38.015925 , LON : -26.780872 , DEPTH : 1601.561 m, TEMP : 4.72163 C, SAL : 35.06863 PSU, DO : 7.79931 mg/l, TURBIDITY : 0.928 FTU [12:29:48] Thanks Mike. [12:30:13] michaelvecchione leaves the room [12:33:53] LAT : 38.015937 , LON : -26.780608 , DEPTH : 1767.3345 m, TEMP : 4.2265 C, SAL : 35.01052 PSU, DO : 7.97746 mg/l, TURBIDITY : 0.928 FTU [12:37:36] good morning all! We are approaching the bottom. Midwater folks - we have 5 niskins set up for eDNA sampling. Do you know which layers you would like to sample in? [12:37:45] kennethsulak leaves the room [12:37:50] Bom dia everyone! [12:38:00] holothurian [12:38:53] LAT : 38.015663 , LON : -26.78154 , DEPTH : 1897.7347 m, TEMP : 3.95247 C, SAL : 34.98539 PSU, DO : 8.00469 mg/l, TURBIDITY : 0.9341 FTU [12:39:06] Allen is our lead on eDNA [12:39:48] Missed eDNA discussion. Stepped aside. [12:40:14] Allen, do we want a sample at 1900m? that's the main question we need answered right now. See above - 5 bottles available. [12:40:18] Solmissus? [12:40:36] llarvacean house [12:40:44] laracean houses [12:40:49] EX2206_DIVE03 ROV on Bottom [12:40:54] @allen i vote yes for an eDNA sample at 1900 [12:41:00] thoughts? [12:41:08] Definitely would like an eDNA sample from down here [12:41:18] Thanks! [12:41:31] johnreed leaves the room [12:41:51] @allen okay great. thinking here and at 1200 for sure based on eDNA sampling last midwater dive? [12:42:04] We will probably also want to catch water in the DSL. [12:42:10] i agree mike [12:42:14] agreed [12:42:18] Yes, in this layer, 1200, DSL, plus 2 more [12:42:24] I vote for 1200 [12:42:33] Adrienne, Mike, and Allen - do you plan on calling in to talk about what we are doing and seeing? [12:42:54] so 1900, 12000, DSL so far [12:43:05] daphnecuvelier leaves the room [12:43:11] I can't talk. Have sleeping family this morning. [12:43:19] I am hoping for Adrienne, but if not I will call in. [12:43:44] isopod [12:43:54] LAT : 38.016166 , LON : -26.781495 , DEPTH : 1897.6935 m, TEMP : 3.9523 C, SAL : 34.98521 PSU, DO : 8.0402 mg/l, TURBIDITY : 0.9341 FTU [12:44:09] okay i will call in haha - mike you should definitely join [12:44:14] holothurian [12:44:22] swimming cuc [12:44:29] larv house [12:44:43] irissampaio leaves the room [12:44:43] many holothurians [12:44:55] tons! [12:45:09] never seen so many [12:47:26] samcandio leaves the room [12:47:27] Hi Isabel! [12:47:45] who was the second person - they were very faint. [12:48:04] @deb Ashley was the second person [12:48:40] Sorry - I'll move my mic closer to my mouth next time i talk haha [12:48:51] thanks! [12:48:52] Hi Allen! [12:48:56] LAT : 38.016253 , LON : -26.781651 , DEPTH : 1897.4101 m, TEMP : 3.96107 C, SAL : 34.98434 PSU, DO : 8.019 mg/l, TURBIDITY : 0.928 FTU [12:49:31] johnreed leaves the room [12:49:47] ctenophore [12:49:56] Ctenophore. Bathocyroe like but dark [12:50:14] elisabettamenini leaves the room [12:50:38] my video locked up just before the ctenophore. [12:50:48] EX2206_DIVE03 Mid-water Transect Start [12:52:56] Atolla? [12:53:02] narcomedusa, [12:53:09] Black forked tail fish is a slickhead - Alepocephalidae. Most are benthopelagic, some are strictly bathypelagic, but others are optional [12:53:20] The bell is the main body [12:53:27] Can we collect this? [12:53:55] Might be too late. It is okay to let go [12:53:57] LAT : 38.016359 , LON : -26.781523 , DEPTH : 1897.3786 m, TEMP : 3.94881 C, SAL : 34.98516 PSU, DO : 7.9967 mg/l, TURBIDITY : 0.9341 FTU [12:54:06] Understood. Can do [12:54:17] johnreed leaves the room: Replaced by new connection [12:54:25] understood thanks deb! [12:55:14] i don't think pilot is on the party line, but if he is let me know so i can stop telling you what he is saying. :) [12:55:41] Yes, looks like nemertean [12:55:45] could be nemertine [12:55:52] Pilots aren't on the party line [12:56:05] @deb the pilot is not on the party line so thank you! [12:56:25] shrimp [12:57:40] That is a black cydippid ctenophore. [12:57:45] ctenophore [12:57:56] thanks mike for correcting that - i totally meant benthic - whoops [12:58:18] beautiful! [12:58:34] Alepocephalids are called slickheads or smoothheads - due to the lack of scales on the head. They are not particularly slimy (except one genus). The common name slimehead is actually used for a totally different family of fishes - the Ateleopodidae. Also used sometimes for species of Hoplostetus [12:58:42] Isopod swimming [12:58:57] LAT : 38.016388 , LON : -26.781562 , DEPTH : 1897.1874 m, TEMP : 3.94958 C, SAL : 34.98444 PSU, DO : 8.03288 mg/l, TURBIDITY : 0.9402 FTU [12:59:08] What genus of isopod did you say that was, Mike? [12:59:12] mmunnopsid isopod [12:59:15] If you spot the black cydippid again, would want it. [12:59:42] Same narco? Looks like. I think it is Cunina. [13:00:04] the black cydippid was at the beginning, yes? The geologist apologizes in advance. [13:00:31] Yes. black cydippid had the two long tentacles deployed [13:00:35] nemertean [13:01:14] I also think it was a larvacean house [13:01:24] thanks tara! [13:02:11] The holothurians swimming with exaggerated undulation may be Epnyniastes eximia - the famous 'headless chicken' or genus Peniagone [13:02:34] not enypniastes [13:02:35] kaseycantwell leaves the room [13:02:47] Could it be Pelagothuria? [13:02:58] not l [13:03:14] not pelagiothuria [13:03:33] Thanks Mike [13:03:57] LAT : 38.016424 , LON : -26.780858 , DEPTH : 1897.3761 m, TEMP : 3.9523 C, SAL : 34.98536 PSU, DO : 8.01775 mg/l, TURBIDITY : 0.928 FTU [13:05:47] Not pelagothuria - that genus is highly modified - looks like a transparent webbed octopod. And it swims by contracting that webbed umbrella [13:06:11] daphnecuvelier leaves the room [13:07:13] Calycophoran siphonophore. [13:08:00] johnreed leaves the room [13:08:15] Bathocyroe [13:08:57] LAT : 38.015938 , LON : -26.781288 , DEPTH : 1897.4592 m, TEMP : 3.95086 C, SAL : 34.98471 PSU, DO : 8.03234 mg/l, TURBIDITY : 0.9463 FTU [13:10:30] larvacean house again. Looks empty. [13:10:35] agree allen [13:11:15] kicking myself for not begging to collect the black cydippid [13:11:43] taraluke leaves the room [13:11:57] we'll find more cool stuff for you, Allen. [13:12:18] :) For the community Deb. [13:12:27] true, true [13:12:43] I think the holothurians may be Amperima [13:12:51] Allen's kingdom for a cydippid? [13:13:43] foram? [13:13:58] LAT : 38.01671 , LON : -26.781062 , DEPTH : 1896.212 m, TEMP : 3.95452 C, SAL : 34.98609 PSU, DO : 8.02177 mg/l, TURBIDITY : 0.9341 FTU [13:14:15] Mike, are those holothurians well collected, documented? [13:14:37] little hydrozoan [13:14:50] allen, i think so [13:14:57] lower right narcomedusa [13:15:12] Another isopod there [13:15:28] So a departure in body plan for those guys [13:15:36] google Amperima event [13:15:37] There are many benthic macrourids that rise off the bottom and drift or scull in midwater. But there are also rattails that are totally adapted to living off bottom - never descending to the substrate [13:16:01] shrimp [13:16:30] i agree mike once they zoomed in [13:17:29] Another cucumber. So still [13:17:54] Wow, great footage [13:18:47] Ugh. Nice. That was another cydippid ctenophore [13:18:49] ctenophore [13:18:58] LAT : 38.016627 , LON : -26.7809 , DEPTH : 1895.8492 m, TEMP : 3.95197 C, SAL : 34.98576 PSU, DO : 8.0022 mg/l, TURBIDITY : 0.9341 FTU [13:19:15] Black body [13:19:47] fish [13:20:26] snitch. [13:20:30] lol [13:20:32] halodsaur [13:20:34] Conventional wisdom is that holothurians that can swim, feed benthically, then lift off to take advantage of bottom currents to drift to a new benthic feeding area. However, I wonder if some do not also feed facultatively in midwater, especially the very active swimmers?? Indeed, the highly-modified genus Pelagothuria never descends to the substrate - so much feed pelagically [13:20:38] Can [13:20:44] 't quite tell. Yes [13:20:58] it is black please [13:22:07] Sorry for being slow to see the black earlier. [13:22:20] thanks for trying pilots! [13:22:30] All good. I think I need to restart vid. May be a bit delayed. [13:23:07] and absolutely make sure everyone is on the full high res feed, not the public feed - I know you all know this. [13:23:11] They have the eyes! [13:23:58] LAT : 38.01685 , LON : -26.780689 , DEPTH : 1895.8873 m, TEMP : 3.95247 C, SAL : 34.9856 PSU, DO : 8.0357 mg/l, TURBIDITY : 0.9341 FTU [13:24:05] I love these benthic boundary layer transects. [13:25:40] I'm not sure what that is. Trachymedusa [13:25:47] But what family. . . [13:26:42] I captured the time stamp for you so I'll send it to you after the dive, Allen [13:26:50] Must have been a rhopalonematid. Crossota probably but tentacles not out [13:26:54] siphonophore [13:27:01] Allen -- Didn't Mike Ford get a black ctenophore from the Caribbean? [13:27:16] lantern shark? [13:27:57] I agree with etmopteridae mike! [13:28:00] possibly? Deffinitely squaliform but couldn't get good enough look to confirm if it was Etmopteridae [13:28:53] look at those fin spines! So big [13:28:59] LAT : 38.016525 , LON : -26.780606 , DEPTH : 1896.2625 m, TEMP : 3.95596 C, SAL : 34.98456 PSU, DO : 8.04547 mg/l, TURBIDITY : 0.928 FTU [13:29:15] @Mike, I do not recall if Ford got a black cteno. [13:29:19] Thanks Roland! [13:30:37] foram [13:30:51] Medusa floated by on left [13:31:08] Get it! Thanks! [13:31:32] Shark was a squaliform, family Somniosidae, either genus Centroscymnus or Scymnodon [13:31:44] Yay! And thanks!~~!!!! [13:31:48] Great hunting. [13:31:52] Thanks, Ken! [13:32:18] jaymesawbrey leaves the room [13:33:23] Great idea Toland [13:33:34] *Roland [13:33:56] I think you're right, Mike [13:34:00] assfish? [13:34:01] LAT : 38.017335 , LON : -26.78086 , DEPTH : 1895.8307 m, TEMP : 3.95308 C, SAL : 34.98498 PSU, DO : 8.00904 mg/l, TURBIDITY : 0.928 FTU [13:34:04] Crossota [13:34:25] mysid? [13:34:31] Bathocyroe again [13:34:35] Gorgeous [13:35:57] Video [13:36:16] Tentacles with tentilla deployed. Fishing for crustaceans [13:36:37] EX2206_DIVE03 Mid-water Transect End [13:36:45] Pulled in fishing gear and took off! [13:36:50] Nice transect. [13:37:12] very benthopelagic [13:38:12] EX2206_DIVE03 ROV Ascending [13:38:16] jaymesawbrey leaves the room [13:38:55] allencollins leaves the room [13:39:00] LAT : 38.016542 , LON : -26.78128 , DEPTH : 1861.8855 m, TEMP : 3.98247 C, SAL : 34.98778 PSU, DO : 8.00594 mg/l, TURBIDITY : 0.928 FTU [13:40:17] kennethsulak leaves the room [13:42:54] johnreed leaves the room [13:43:54] taraluke leaves the room [13:44:01] LAT : 38.016515 , LON : -26.781149 , DEPTH : 1709.9258 m, TEMP : 4.34645 C, SAL : 35.02274 PSU, DO : 7.96899 mg/l, TURBIDITY : 0.928 FTU [13:44:18] allencollins leaves the room [13:44:52] Mike - that gray-brown bulbous head fish that looked like a cusk-eel is actually a highly aberrant macrourid, Squalogadus. Sorry for late ID - got bumped off the feed [13:48:12] Thanks, Ken! I'll go switch the annotation [13:48:42] allencollins leaves the room [13:49:01] LAT : 38.016556 , LON : -26.781018 , DEPTH : 1555.8424 m, TEMP : 4.90244 C, SAL : 35.0858 PSU, DO : 7.73747 mg/l, TURBIDITY : 0.9402 FTU [13:51:22] Thanks Ken. I have been having trouble with the feed today too, but I am out at my cabin in the woods so I assume mine is a local problem. [13:51:53] Squalogadus lacks a first dorsal fin - only such macrourid I think. Big inflated head is a floatation device. Sometimes this fish is seen close to the seafloor, but may be more typically deep pelagic. [13:52:46] Mike - same here - rural location with funky DSL that fails frequently [13:53:56] jaymesawbrey leaves the room [13:54:01] LAT : 38.016989 , LON : -26.780816 , DEPTH : 1405.2532 m, TEMP : 5.63385 C, SAL : 35.17215 PSU, DO : 7.42109 mg/l, TURBIDITY : 0.928 FTU [13:54:57] daphnecuvelier leaves the room [13:55:30] taraluke leaves the room [13:55:44] Note on distinquishing Etmopterus lantern sharks from closely-related velvet dogsharks. Etmopterus has a lower, longer caudal fin almost in line with main body axis. Centroscymnus and Scymnodon and related genera placed in family Somniosidae now are fatter midsection and have a kind of shortish diamond shaped caudal fin [13:57:12] My preferred solution is to ask a fish person. [13:57:16] Thanks! I was just trying to pull up a key to the genera to figure that out haha [13:57:23] Otherwise general body shape and fin position is similar in both groups of deep-living black dogsharks [13:58:50] allencollins leaves the room [13:59:02] LAT : 38.017237 , LON : -26.780742 , DEPTH : 1255.2451 m, TEMP : 6.84003 C, SAL : 35.36311 PSU, DO : 6.87108 mg/l, TURBIDITY : 0.9402 FTU [13:59:45] words of wisdom for differentiating the Squalogadus from other fish that look similar (like ophidids and liparids)? [14:00:33] cyclothone [14:02:27] cyclothone [14:02:57] Ashley - very few other fishes look like that big bulbous headed tadpole-like Squalogadus - small nostrils, large eyes. Only somewhat similar fish is the blind cusk eel Typhlonus nasus - large nostrils, no eyes [14:03:41] cyclothone? [14:03:49] yup that was a cyclothone [14:03:57] okay, thanks Ken! [14:04:02] LAT : 38.017902 , LON : -26.779881 , DEPTH : 1200.3103 m, TEMP : 7.14514 C, SAL : 35.38849 PSU, DO : 6.71041 mg/l, TURBIDITY : 0.9463 FTU [14:04:26] Squalogadus has a very ventral mouth. I think it emulates swimming holos - drifting/sculling off-bottom, using off-bottom currents to move to a new benthic feeding location, descending periodically [14:04:43] Ashley, sisnce you and Ken are on I am going to stop pretending to know the fishes. [14:05:08] you're faster with a lot of these IDs then I am, Mike haha [14:05:28] taraluke leaves the room [14:05:34] I'm so busy annotating that I don't get a chance to stare at the fish so you're tentative IDs are actually very helpful! [14:06:00] daphnecuvelier leaves the room [14:06:10] ok [14:06:42] cyclo [14:07:43] johnreed leaves the room [14:08:10] Hi Nick! [14:08:43] We are missing Dhugal [14:08:53] How much holothurian DNA is going to be in that water sample from the first transect? [14:09:03] LAT : 38.017978 , LON : -26.779629 , DEPTH : 1201.2068 m, TEMP : 7.15305 C, SAL : 35.39059 PSU, DO : 6.69596 mg/l, TURBIDITY : 0.9402 FTU [14:09:17] EX2206_DIVE03 Mid-water Transect Start [14:09:39] holothurian ground truth of eDNA [14:10:21] Hey midwater folks - there was an accidental holothurian collection in the 1990m transect. it is currently in one of our suction canisters. are you ok with keeping it? I might be able to ask them to flush it... [14:10:32] sorry - 1900m [14:10:55] allencollins leaves the room [14:11:13] we still have 3 empty canisters, correct? [14:11:22] yes, three empty canisters [14:11:29] @mike - do you know anyone that would want the benthic holothurian? [14:11:31] Deborah, can you wait to flush until all of the other canisters are full? [14:11:32] kennethsulak leaves the room [14:11:56] I suspect it is not very valuble. [14:12:05] yes, i can certainly wait. i can check to make sure it's possible or not too. [14:12:08] I'm sure Chris Mah wouldn't turn down a benthic holothurian [14:12:17] haha [14:13:29] jelly top center [14:13:33] foram and chaetognath [14:14:03] LAT : 38.018133 , LON : -26.779643 , DEPTH : 1201.025 m, TEMP : 7.15775 C, SAL : 35.38977 PSU, DO : 6.70633 mg/l, TURBIDITY : 0.9463 FTU [14:15:36] johnreed leaves the room [14:16:13] There is just a single Amperima (swimming holothuroid) barcode in Genbank. So, that sample could be useful. [14:17:25] jaymesawbrey leaves the room [14:18:06] mucus [14:18:12] mucus stroing [14:18:16] string [14:18:40] probably a discarded tentacle from a siphonophoe [14:18:45] nemertean? [14:18:56] Polychaete [14:19:00] o chaetognath [14:19:02] polychaet [14:19:05] LAT : 38.018229 , LON : -26.779519 , DEPTH : 1201.5073 m, TEMP : 7.15664 C, SAL : 35.38689 PSU, DO : 6.71866 mg/l, TURBIDITY : 0.9341 FTU [14:19:09] haha i mean polychaete [14:19:16] Definitely polychaete once we zoomed in [14:19:25] taraluke leaves the room [14:19:28] PS, don't use the word "mucus? [14:19:37] "at the diner table [14:20:16] sorry my video keeps freezing today [14:20:33] chaetognath [14:20:39] Huh. Keep videoing please [14:20:44] chaet [14:20:47] JavierCristobo leaves the room [14:20:48] arrow worm [14:21:07] not typically that red color though [14:21:26] cyclo [14:21:40] a type of bristlemouth [14:21:59] most abundant vertebrate on planet. Measured in quadrillions [14:22:16] some bathypelagic chaetognaths are red [14:22:23] megancromwell leaves the room [14:22:35] I don't think it's a polychaete [14:22:56] so we could flush the holoturian from suction canister 1 if you need to. we can hold on to it until you make a decision. but we'd probably want to flush it well before you needed that last sample... [14:23:09] as a reminder, it was an accidental sample. [14:23:15] thanks deb! [14:24:04] LAT : 38.018341 , LON : -26.779403 , DEPTH : 1200.4566 m, TEMP : 7.11658 C, SAL : 35.38567 PSU, DO : 6.71 mg/l, TURBIDITY : 0.9585 FTU [14:24:09] I should note that Amperima (holothurian) was just guess on my part. [14:24:16] I think we can hold on to it until we fill up 2 more canisters at least [14:24:31] I agree @Ashley [14:24:50] pteropod? [14:25:01] Limacina helicina [14:25:20] wait wrong species [14:25:26] Calycophoran siph [14:25:28] syphonophore [14:25:44] Different from the one before. [14:26:24] That was a nice view of the siph. A screen capture could be used for refined ID. [14:26:47] I'll get the timestamp for you, Allen [14:27:00] good idea allen - @isabel do you want to mark this one in the annotations for review? [14:27:04] holothurian [14:27:06] Maybe a foram? [14:27:11] Or rad [14:27:49] Next time that medusa will be in the center [14:28:44] Great view on that larvacean [14:29:05] LAT : 38.01853 , LON : -26.779232 , DEPTH : 1200.8154 m, TEMP : 7.12629 C, SAL : 35.38612 PSU, DO : 6.72 mg/l, TURBIDITY : 0.9402 FTU [14:30:21] batycyroe ctenophore [14:30:46] bathocyroe [14:31:31] johnreed leaves the room [14:32:36] shrimp [14:32:40] mysid? [14:32:53] not mysid [14:32:57] agreed [14:32:59] daphnecuvelier leaves the room [14:34:05] LAT : 38.018795 , LON : -26.77909 , DEPTH : 1200.656 m, TEMP : 7.13564 C, SAL : 35.38705 PSU, DO : 6.70188 mg/l, TURBIDITY : 0.9402 FTU [14:34:09] As Mike said earlier, the heavily pigmented stomach keeps prey from giving away their position while bioluminescing in their stomach. Another interesting point, the next time we see one is that red is a good color because most organisms that can see in the deep sea don't see the color red, so it makes them extra-invisible. [14:34:34] yep mucus [14:34:54] Bathocyroe. Can get nice footage of it [14:35:03] Frog kicking away! [14:35:13] ha [14:35:21] Chaet [14:35:50] Nice comment Candio! [14:35:57] The pteropod was Limacina helicina (I should hve trusted my initial guess). [14:36:59] Ctenophores, especially those lobates are really delicate. Nearly impossible to preserve well. [14:37:04] ctenophore [14:37:04] michaelvecchione leaves the room [14:37:12] Exactly. The video is hugely important [14:37:49] most of the samples in the past were from trawl nets which destroy these gelatinous fauna [14:38:41] isabelmoyer leaves the room [14:38:41] another cyclothone? [14:38:46] yup [14:38:52] michaelvecchione leaves the room [14:39:02] thanks ashley - my video froze again [14:39:07] LAT : 38.019015 , LON : -26.778916 , DEPTH : 1202.2576 m, TEMP : 7.07849 C, SAL : 35.37885 PSU, DO : 6.72653 mg/l, TURBIDITY : 0.9402 FTU [14:40:05] speaking of ctenophores, If I could make a request if thats okay, for the ctenophore that was collected earlier if someone has time I would love some really closeup shots of the animal as they dont preserve well and would help with ID. If not no worries any picture's will be amazing help [14:40:20] The black ctenophore will hold up for a bit under the photography set up if kept cold. Maybe 10 minutes or so. If you can get images with very strong sidelighting, it will be very helpful. [14:41:16] @allen, we will do our best [14:41:19] This is great. A lot of hidden diversity in this genus Bathocyroe, but this is reasonably close to type locality for B. fosteri. [14:41:37] is there a maximum time we can keep it before it degrades? [14:41:42] @Joana, I know you will! And whatever is possible is enough. ;-) [14:42:19] ya they must be kept cold or their body starts to disintigrate like the witch in the wizard of oz [14:42:25] Shrimp, same species as before [14:42:45] taraluke leaves the room [14:43:11] @mike, can you write the shrimp species here, if you know? I'm just back from lunch so may have missed it [14:43:19] @joana, there is variability. One cydippid I was looking at on board started disintegrating within 5-10 minutes of photographing. It started to change shape, then the combs started to break off. If you see that starting, pop into perservative [14:43:42] Not sure on species, will send off to Tammy Frank and Heather Bracken-Grissom for IDs after the dive though [14:44:07] LAT : 38.019246 , LON : -26.778703 , DEPTH : 1200.1675 m, TEMP : 6.99004 C, SAL : 35.36672 PSU, DO : 6.78148 mg/l, TURBIDITY : 0.9341 FTU [14:44:51] discarded larvacean house [14:45:49] an Allen animal [14:46:26] That was halicreas minima [14:46:34] michaelvecchione leaves the room [14:46:49] @allen, ok, we will do that. We will keep it in cool water and process it asap [14:47:17] arroworm [14:48:34] michaelvecchione leaves the room [14:49:07] LAT : 38.019527 , LON : -26.778443 , DEPTH : 1199.7831 m, TEMP : 6.95506 C, SAL : 35.36344 PSU, DO : 6.77111 mg/l, TURBIDITY : 0.9402 FTU [14:49:27] once we've got all samples collected you can tell us which ones we should process first [14:50:23] Thanks Joana. If there is loose tentacle on the cydippid, that can serve as the subsample for genetics. Or if you can pick some of the tentacle off, that would work. The remainder of animal can go into formalin, and with fingers crossed we will get something to actually examine for morphology. And a huge thank you. [14:50:59] bathycyroe [14:51:34] roger that @allen [14:52:00] isabelmoyer leaves the room [14:54:00] All the picoplankton [14:54:04] siph [14:54:08] LAT : 38.019703 , LON : -26.778402 , DEPTH : 1200.6603 m, TEMP : 6.96506 C, SAL : 35.36482 PSU, DO : 6.77489 mg/l, TURBIDITY : 0.928 FTU [14:54:10] Yes, exactly Joana! [14:54:22] rocket ship siphonophore [14:54:31] EX2206_DIVE03 Mid-water Transect End [14:54:48] Most likely family Diphyidae [14:55:17] EX2206_DIVE03 ROV Ascending [14:55:40] OK. Other than water, no collection. 3 cannisters empty and waiting. :) [14:55:46] allen, what's the distinguishing feature of Calcophorins? [14:57:15] Swimming bells without a "float"/pneumatophore. [14:58:32] If you just have a pneumatophore (like Physalia the Port. man-o-war) it is Cystonectae. [14:58:37] @allen, you guys let us know and we will do our best to bring all of those canisters with a "jello" :-) [14:59:09] LAT : 38.019036 , LON : -26.778743 , DEPTH : 1172.442 m, TEMP : 7.10575 C, SAL : 35.38375 PSU, DO : 6.71999 mg/l, TURBIDITY : 0.9402 FTU [14:59:35] IF both float and swimming bells, then it is part of a "grade" that used to be the taxon Physonectae. The calycophorans diverged from within the physonects. [14:59:37] taraluke leaves the room [15:00:05] isabelmoyer leaves the room [15:00:31] johnreed leaves the room [15:01:28] okay! [15:03:46] but I liked Physonectae [15:04:03] daphnecuvelier leaves the room [15:04:09] LAT : 38.019093 , LON : -26.778553 , DEPTH : 1028.3165 m, TEMP : 8.51204 C, SAL : 35.55992 PSU, DO : 6.16407 mg/l, TURBIDITY : 0.9402 FTU [15:04:52] michaelvecchione leaves the room [15:05:41] nolanbarrett leaves the room [15:06:14] michaelvecchione leaves the room [15:08:13] @shore science team -- our remaining transects are : 900, 700, 500, 300 we currently do not have time left in the dive to do a seperate DSL transect beyond these. Would you like to move one of these transects into the DSL? [15:08:16] allencollins leaves the room [15:08:20] DSL is currently at 600 m [15:09:00] we will need an answer prior to the end of the next transect (900 m) [15:09:04] @kasey can you pull up the Eks on the camera 3? [15:09:10] LAT : 38.019091 , LON : -26.778454 , DEPTH : 901.2801 m, TEMP : 9.68914 C, SAL : 35.67155 PSU, DO : 5.66556 mg/l, TURBIDITY : 0.9463 FTU [15:09:57] michaelvecchione leaves the room [15:12:02] megancromwell leaves the room [15:12:24] I think my message about transects got dropped. My preference is to drop the 300 and add DSL (i.e., 900, 700, 600 DSL, 500) [15:12:25] taraluke leaves the room [15:12:43] daphnecuvelier leaves the room [15:13:18] cyclo [15:13:44] @sam can you show us the top, bottom, and highest areas of the DSL? [15:13:59] it seems like it is spanning a very large depth range on the EKs actually [15:14:04] yes, stand by 1 [15:14:09] thanks! [15:14:11] LAT : 38.019723 , LON : -26.778418 , DEPTH : 899.5323 m, TEMP : 9.71066 C, SAL : 35.6715 PSU, DO : 5.658 mg/l, TURBIDITY : 0.9463 FTU [15:14:51] michaelvecchione leaves the room [15:15:24] EX2206_DIVE03 Mid-water Transect Start [15:15:46] okay thanks sam - you can go back to the normal screen now [15:16:00] sounds good! [15:16:04] looking at the EKs I am fine with Mike's suggestions [15:16:21] so doing transects at 900, 700, 600 , and 500 [15:16:31] agreed [15:16:35] seems like both 500 and 600 will be in the DSL [15:16:49] and 700 right below which will be interesting [15:17:17] cyclothone [15:17:24] k cool [15:19:10] LAT : 38.019804 , LON : -26.778512 , DEPTH : 900.9062 m, TEMP : 9.75796 C, SAL : 35.67232 PSU, DO : 5.6561 mg/l, TURBIDITY : 0.9463 FTU [15:19:11] We only have 2 niskins left, correct? [15:19:25] nolanbarrett leaves the room [15:19:37] cyclo [15:20:23] 2 niskins left correct [15:20:51] anyone want to join us on the line? [15:21:06] foram [15:21:16]